AA-Ac Labeling Kit

Part number: LT-KAAC-A2

AA-Ac was developed for rapid profiling of complex oligosaccharide mixtures [Charlwood et al (2000)].

AA-Ac labeled oligosaccharides can be analysed by HPLC, MALDI-MS, free-zone capillary electrophoresis, and capillary electrophoresis / electrospray ionization mass spectrometry [Charlwood et al (2000)].

AA-Ac is a versatile fluorescent probe for glycan analysis. It is superior to its predecessor AMAC (2-aminoacridone) with regard to fluorescence intensity and replaces AMAC for many applications. The latter probe has been used in a variety of studies including those on human milk glycans [Charlwood et al (1999(], human transferrrin [Charlwood et al (1998)], immunoglobulins [Okafo et al (1996)], and glycosylaminoglycans [Kitagawa et al (1995)].

The LudgerSep N1 column (Cat # LS-N1-4.6x250) is an excellent choice for amide HPLC profiling and fingerprinting of complex AA-Ac-labeled glycan mixtures.

The kit contains two sets of the following reagents (supplied in glass ampoules sealed under pure nitrogen):

• 3-(acetylamino)-6-aminoacridine (AA-Ac dye)
• Dimethyl sulfoxide (DMSO)
• Acetic acid
• Sodium cyanoborohydride

Purity: > 99% by HPLC. Soluble in DMSO and ethanol. M.W. 278. lambda-ex = 382 or 445 nm, lambda-em = 525 nm.

1. Charlwood, J.; Birrell, H. Gribble, A.; Burdes, V.; Tolson, D.; Camilleri, P. (2000)
   A probe for the versatile analysis and characterization of N-linked oligosaccharides.
   Analytical Chemistry 72: 1453-1461



2. Charlwood, J. et al (1999)
   Analytical Biochemistry 273: 261-277
   



3. Charlwood, J. et al (1998)
   Analytical Chemistry 70:2530-2535
   



4. Okafo, G.N. et al (1996)
   Analytical Biochemistry 240: 68-74
   



5. Kitagawa, H et al (1995)
   Analytical Biochemistry 232: 114-121
   

Part number: LT-KAAC-A2