Oligosaccharide Profiling

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	Oligosaccharide Profiling
		N-linked Oligosaccharide Profiling-Fluorescent
		N-linked Oligosaccharide Profiling-HPAEC
		O-linked Oligosaccharide Profiling
		MALDI-TOF Mass Spec
	Monosaccharide Analysis
	Sialic Acid Analysis

Oligosaccharide Profiling

Oligosaccharide profiling provides a qualitative profile, or map, of the various oligosaccharides in a sample. This is a very powerful method for comparing the glycosylation of different samples.

N-linked Oligosaccharide Profiling-Fluorescent

N-linked oligosaccharides are released from the glycoprotein using the enzyme PNGase F

The released glycans are labeled with a fluorophore and then separated by HPLC.

The labeled oligosaccharides are separated by normal phase chromatography which separates the oligosaccharides into groups according to their negative charge.

Versions of this method that has been optimized for recombinant monoclonal antibodies (MAbs), in addition to other biotherapeutics such as erythropoietin and various blood factors.

N-linked Oligosaccharide Profiling-HPAEC

N-linked oligosaccharides are released from the glycoprotein using PNGase F

Unlabeled glycans are analyzed by HPLC.

Oligosaccharides are separated by anion exchange chromatography (HPAEC), separating glycans according to negative charge.

As with our fluorescent methods, assays have been optimized for antibodies and many other glycoproteins.

O-linked Oligosaccharide Profiling

O-linked oligosaccharides are released from the glycoprotein chemically using alkaline degradation

Unlabeled oligosaccharides are separated by anion exchange chromatography

Glycans are separated based on negative charge.

This assay is specific for oligosaccharides linked to the amino acids serine or threonine in proteins, referred to as O‑linked oligosaccharides or O-glycans.

MALDI-TOF Mass Spec

In this analysis, oligosaccharides are released from the glycoprotein and then analyzed by mass spectrometry. Masses are determined for the different oligosaccharide structures in the pool.